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Contents

10/09/2013 00:16

Know evrything here from what is DNA to how it is manipulated in the world of RDT.

DNA

  • Discovery of DNA

  • DNA Structure

RDT

  • How it all started..and what it is all about

  • An overview of RDT and the steps involved

Tools of RDT

  • Restriction Endonuclease

  • Other important ENZYMES used in RDT..

  • Vectors

Gene Cloning

  • DNA Extraction

        -Purification of DNA from living cells

        -Total cell DNA

        -Plasmid DNA

        -Phage DNA

  • Agarose Gel Electrophoresis

  • Preparation of insert and vector DNA

  • Polymerase Chain Reaction(PCR)

  • DNA Ligation(vector and insert)

  • Transfer of DNA into host cell

         -An introduction.. cell based DNA cloning

         -Transfer of DNA into host cells-the various methods

  • Screening of cloned genes

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    • Tools of RDT
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recdnatech

kalsri140993@gmail.com

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