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Contents

10/09/2013 00:16

Know evrything here from what is DNA to how it is manipulated in the world of RDT.

DNA

Discovery of DNA
DNA Structure

RDT

Tools of RDT

Restriction Endonuclease
Other important ENZYMES used in RDT..
Vectors

Gene Cloning

DNA Extraction

-Purification of DNA from living cells

-Total cell DNA

-Plasmid DNA

Agarose Gel Electrophoresis
Preparation of insert and vector DNA
Polymerase Chain Reaction(PCR)
DNA Ligation(vector and insert)
Transfer of DNA into host cell

-An introduction.. cell based DNA cloning

-Transfer of DNA into host cells-the various methods

Screening of cloned genes

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