DNA Discovery

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DNA Structure

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RDT(overview)

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Restriction Endonuclease

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Important Enzymes used in RDT

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Vectors

/album/vectors/fdfd-jpg1/ Plasmid /album/vectors/a400px-plasmid-replication-english-svg-png1/ /album/vectors/a250px-conjugation-svg-png1/ /album/vectors/pbr322a-jpeg1/ pBR322
/album/vectors/mga2-08-10-puc18-jpg1/ pUC18 /album/vectors/a19-06lambdalyticlysocycle-l-jpg1/ Infection by Bacteriophage /album/vectors/f-jpg/ /album/vectors/f2-008-jpg1/ M13 phage infection

DNA Extraction

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/album/dna-extraction1/images-2-jpg1/ Ethidium bromide-caesium chloride density gradient centrifugation

Agarose gel electrophoresis

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Polymerase Chain Reaction(PCR)

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Ligation(vector and insert)

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Cell based DNA cloning

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Gene cloning

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Transfer of DNA into host cell

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/album/transfer-of-dna-into-host-cell/download-1-jpg1/ Gene gun