DNA Extraction
Purification of DNA from living cells
08/09/2013 19:22
he genetic engineer will, at different times, need to prepare at least three distinct kinds of DNA.
Firstly, total cell DNA will often be required as a source of material from which to obtain genes to be cloned.
Total cell DNA may be DNA from a culture of bacteria,...
Total cell DNA
06/09/2013 19:24
Preparation of total cell DNA
The fundamentals of DNA preparation are most easily understood by first considering the simplest type of DNA purification procedure, that wbere the entire DNA complement of a bacterial cell is required. The modifications needed for plasmid and phage DNA...
Plasmid DNA
06/09/2013 19:12
Preparation of plasmid DNA
Purification of plasmids from a culture of bacteria involves the same general strategy as preparation of total cell DNA.
A culture of cells, containing plasmids, is grown in liquid medium, harvested, and a cell extract prepared. The protein and RNA are...
Phage DNA
05/09/2013 19:26
Preparation of bacteriophage DNA
The key difference between phage DNA purification and the preparation of either total cell DNA or plasmid DNA is that for phages the starting material is not normally a cell extract. This is because bacteriophage particles can be obtained in large...