Ligation of vector and insert

DNA Ligation(vector and insert)

11/09/2013 20:27
Ligation The next step is the ligation of the insert into the linearized vector. This involves the formation of phosphodiester bonds between adjacent 5'-phosphate and 3'-hydroxyl residues, which can be catalyzed by two different ligases: E. coli DNA ligase and bacteriophage T4 DNA...