Contents

10/09/2013 00:16

Know evrything here from what is DNA to how it is manipulated in the world of RDT.

DNA

• Discovery of DNA

• DNA Structure

RDT

• How it all started..and what it is all about

• An overview of RDT and the steps involved

Tools of RDT

• Restriction Endonuclease

• Other important ENZYMES used in RDT..

• Vectors

Gene Cloning

• DNA Extraction

        -Purification of DNA from living cells

        -Total cell DNA

        -Plasmid DNA

        -Phage DNA

• Agarose Gel Electrophoresis

• Preparation of insert and vector DNA

• Polymerase Chain Reaction(PCR)

• DNA Ligation(vector and insert)

• Transfer of DNA into host cell

         -An introduction.. cell based DNA cloning

         -Transfer of DNA into host cells-the various methods

• Screening of cloned genes